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noti-flanked minitransposon encoding the kanamycin resistance cassette m1-kan r  (Thermo Fisher)


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    Structured Review

    Thermo Fisher noti-flanked minitransposon encoding the kanamycin resistance cassette m1-kan r
    Noti Flanked Minitransposon Encoding The Kanamycin Resistance Cassette M1 Kan R, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/kan+r+cassette/noti+flanked+minitransposon+encoding+the+kanamycin+resistance+cassette+m1+kan+r/pmc06858789-478-3-13
    Average 90 stars, based on 1 article reviews
    noti-flanked minitransposon encoding the kanamycin resistance cassette m1-kan r - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Isolation:

    Article Title: Stable Preanaphase Spindle Positioning Requires Bud6p and an Apparent Interaction between the Spindle Pole Bodies and the Neck
    Article Snippet: .. Transformants that had successfully integrated the cnm67 :: kan R cassette were isolated based on (i) their ability to grow in media supplemented with G418 (200 mg/liter; Invitrogen, Carlsbad, CA) and (ii) PCR analysis to verify heterozygosity for the CNM67 loci ( CNM67/cnm67 :: kan R ). ..

    Polymerase Chain Reaction:

    Article Title: Stable Preanaphase Spindle Positioning Requires Bud6p and an Apparent Interaction between the Spindle Pole Bodies and the Neck
    Article Snippet: .. Transformants that had successfully integrated the cnm67 :: kan R cassette were isolated based on (i) their ability to grow in media supplemented with G418 (200 mg/liter; Invitrogen, Carlsbad, CA) and (ii) PCR analysis to verify heterozygosity for the CNM67 loci ( CNM67/cnm67 :: kan R ). ..

    Article Title: A MultiSite Gateway Toolkit for Rapid Cloning of Vertebrate Expression Constructs with Diverse Research Applications
    Article Snippet: .. Unless otherwise stated, all 3’ entry vectors were generated by PCR amplification of the desired 3’ element using attB2R/B3-flanked oligonucleotide primers, followed by a BP reaction with pDONR P2R-P3 (Invitrogen). p3E-GFP-HA, p3E-YFP-HA (from pEYFP-C1, Clontech), p3E-CFP-HA (from pECFP-C1, Clontech), p3E-mCherry-HA (from p3E-mCherrypA [ ]), p3E-mKate2-HA and p3E-mKate2-myc no-polyadenylation signal (pA) were made by amplification of the coding sequences without a stop codon, but with the 3’ primer additionally containing an HA or c-myc epitope sequence followed by a stop codon. p3E-HA-Neuroligin1 was generated by amplification of HA-Neuroligin1 from a previously described vector [ ]. p3E-Dam-myc no-pA is from pNDam-Myc [ ]. p3E-SGTAP no-pA originated from pCeMM-CTAP(SG)-Gw [ ]. p3E-FRT-Kan R -FRT pA was cloned by amplifying the Kan R cassette from pCRII (ThermoFisher) with FRT sites in the 5’ and 3’ primers and inserting into the BamHI site of p3E-polyA [ ]. .. FRB variants and FKBP cassettes were amplified from Gerald Crabtree lab plasmids [ – ] to generate p3E-FRB-HA, p3E-FRB(PLF)-HA, p3E-FRB(KTF)-HA, p3E-FKBP-HA and p3E-mCherryFRB-HA no-pA. p3E-HA was made by cloning annealed oligos (5’-GATCCACTCGAGTATCCGTACGACGTACCAGACTACGCAGCATAGGGTACCAGTACTAAC-3’ and 5’-AATTGTTAGTACTGGTACCCTATGCTGCGTAGTCTGGTACGTCGTACGGATACTCGAGTG-3’) into BamHI/MfeI-digested p3E-polyA.

    Transfection:

    Article Title: HVT-delivered chicken IGF-1 improves growth performance in broilers
    Article Snippet: .. The GS1783 cells harboring the pHVT-BAC-Kan-chIGF-1 was treated with l-arabinose at 32°C for 1 h followed by induction at 42°C to induce the I-SceI and remove the Kan R cassette and get the target infectious clone pHVT-BAC-chIGF-1. rHVT-chIGF-1 was rescued by transfected the pHVT-BAC-chIGF-1 into CEFs using LipofectamineTM 3000 reagent (ThermoFisher, USA). ..

    Cell Surface Hydrophobicity:

    Article Title: A Lambda-evo (λ evo ) phage platform for Zika virus E DIII protein display
    Article Snippet: .. Escherichia coli K-12 derivative strains Genotype Reference DH5α supE44 D(lacIZYA-argF)U169 deoR (Φ80lacZDM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 Hanahan 1983 DH5α(λ) λ prophage This work DH5α(λΔD::KanR) λΔD::KanR prophage This work DH5α(λΔD) λΔD prophage This work W3110 F- λ - rph- Bachmann 1972 JW2787 F- Δ(araD-araB)567 ΔlacZ4787(::rrnB- 3) λ ΔrecD744::kan rph-1 Δ(rhaD-rhaB)568 hsdR514 Baba et al. 2006 SoluBL21† F- ompT hsdSB (rB- mB-) gal dcm (DE3) Deatherage et al. 2012 Bacteriophages/viral strains Genotype Reference λwt Lambda wild type CSH Collection λcI857 Lambda tscI857 CSH Collection λΔD::KanR λcI857 with a Kan R cassette inserted in D site This work λΔD λcI857 ΔD This work λevo λΔD with a 10,793 bp deletion in b region This work ZIKV Zika virus MEX_ENCB165P4 This work Plasmid Relevant markers Reference pJET1.2/blunt Eco471R (AmpR) Thermo Fisher Scientific 2024 pJET-ZEDIII-6xHis pJET1.2/blunt with ZEDIII coding sequence and 6xHis Tag (AmpR) This work pJET-Dλ-6xHis pJET1.2/blunt with Dλ (Amp R) This work pJET-Dλ pJET1.2/blunt with Dλ and 6xHis Tag (Amp R) This work pJET-Dλ-ZEDIII-L-6xHis pJET1.2/blunt with Dλ-ZEDIII-L fusion and 6xHis Tag This work pKQV4 Ptac promoter (Amp R) Strauch et al. 1989 pDλ-ZEDIII-6xHis pKQV4 with Dλ-ZEDIII fusion and 6xHis Tag (Amp R) This work pDλ-ZEDIII-L-6xHis pDλ-ZEDIII-6xHis with long linker (Amp R) This work pDλ-ZEDIII pKQV4 with Dλ-ZEDIII (Amp R) This work pDλ-TD-6xHis pKQV4 with Dλ fused to 3 different epitopes from EZIKV, separated by short linkers and 6xHis Tag (AmpR) This work pDλ-GFP pKQV4 with Dλ-GFP (Amp R) This work pDλ-GFP-L pDλ-GFP with long linker (Amp R) This work pDλ pKQV4 with Dλ (Amp R) This work pDλ-6xHis pKQV4 with Dλ and 6xHis Tag (Amp R) This work pKD46 pBAD promoter exo beta gam genes (CmR) Datsenko and Wanner 2000 pE-FLP pBAD promoter flp gene (CmR) Datsenko and Wanner 2000 pRSET-ZIKV/ES pRSET-A with EZIKV coding sequence corresponding to residues 1 to 409 (AmpR) This work † The SoluBL21 strain contains uncharacterized mutations which were obtained through special selection criteria. ..

    Virus:

    Article Title: A Lambda-evo (λ evo ) phage platform for Zika virus E DIII protein display
    Article Snippet: .. Escherichia coli K-12 derivative strains Genotype Reference DH5α supE44 D(lacIZYA-argF)U169 deoR (Φ80lacZDM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 Hanahan 1983 DH5α(λ) λ prophage This work DH5α(λΔD::KanR) λΔD::KanR prophage This work DH5α(λΔD) λΔD prophage This work W3110 F- λ - rph- Bachmann 1972 JW2787 F- Δ(araD-araB)567 ΔlacZ4787(::rrnB- 3) λ ΔrecD744::kan rph-1 Δ(rhaD-rhaB)568 hsdR514 Baba et al. 2006 SoluBL21† F- ompT hsdSB (rB- mB-) gal dcm (DE3) Deatherage et al. 2012 Bacteriophages/viral strains Genotype Reference λwt Lambda wild type CSH Collection λcI857 Lambda tscI857 CSH Collection λΔD::KanR λcI857 with a Kan R cassette inserted in D site This work λΔD λcI857 ΔD This work λevo λΔD with a 10,793 bp deletion in b region This work ZIKV Zika virus MEX_ENCB165P4 This work Plasmid Relevant markers Reference pJET1.2/blunt Eco471R (AmpR) Thermo Fisher Scientific 2024 pJET-ZEDIII-6xHis pJET1.2/blunt with ZEDIII coding sequence and 6xHis Tag (AmpR) This work pJET-Dλ-6xHis pJET1.2/blunt with Dλ (Amp R) This work pJET-Dλ pJET1.2/blunt with Dλ and 6xHis Tag (Amp R) This work pJET-Dλ-ZEDIII-L-6xHis pJET1.2/blunt with Dλ-ZEDIII-L fusion and 6xHis Tag This work pKQV4 Ptac promoter (Amp R) Strauch et al. 1989 pDλ-ZEDIII-6xHis pKQV4 with Dλ-ZEDIII fusion and 6xHis Tag (Amp R) This work pDλ-ZEDIII-L-6xHis pDλ-ZEDIII-6xHis with long linker (Amp R) This work pDλ-ZEDIII pKQV4 with Dλ-ZEDIII (Amp R) This work pDλ-TD-6xHis pKQV4 with Dλ fused to 3 different epitopes from EZIKV, separated by short linkers and 6xHis Tag (AmpR) This work pDλ-GFP pKQV4 with Dλ-GFP (Amp R) This work pDλ-GFP-L pDλ-GFP with long linker (Amp R) This work pDλ pKQV4 with Dλ (Amp R) This work pDλ-6xHis pKQV4 with Dλ and 6xHis Tag (Amp R) This work pKD46 pBAD promoter exo beta gam genes (CmR) Datsenko and Wanner 2000 pE-FLP pBAD promoter flp gene (CmR) Datsenko and Wanner 2000 pRSET-ZIKV/ES pRSET-A with EZIKV coding sequence corresponding to residues 1 to 409 (AmpR) This work † The SoluBL21 strain contains uncharacterized mutations which were obtained through special selection criteria. ..

    Plasmid Preparation:

    Article Title: A Lambda-evo (λ evo ) phage platform for Zika virus E DIII protein display
    Article Snippet: .. Escherichia coli K-12 derivative strains Genotype Reference DH5α supE44 D(lacIZYA-argF)U169 deoR (Φ80lacZDM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 Hanahan 1983 DH5α(λ) λ prophage This work DH5α(λΔD::KanR) λΔD::KanR prophage This work DH5α(λΔD) λΔD prophage This work W3110 F- λ - rph- Bachmann 1972 JW2787 F- Δ(araD-araB)567 ΔlacZ4787(::rrnB- 3) λ ΔrecD744::kan rph-1 Δ(rhaD-rhaB)568 hsdR514 Baba et al. 2006 SoluBL21† F- ompT hsdSB (rB- mB-) gal dcm (DE3) Deatherage et al. 2012 Bacteriophages/viral strains Genotype Reference λwt Lambda wild type CSH Collection λcI857 Lambda tscI857 CSH Collection λΔD::KanR λcI857 with a Kan R cassette inserted in D site This work λΔD λcI857 ΔD This work λevo λΔD with a 10,793 bp deletion in b region This work ZIKV Zika virus MEX_ENCB165P4 This work Plasmid Relevant markers Reference pJET1.2/blunt Eco471R (AmpR) Thermo Fisher Scientific 2024 pJET-ZEDIII-6xHis pJET1.2/blunt with ZEDIII coding sequence and 6xHis Tag (AmpR) This work pJET-Dλ-6xHis pJET1.2/blunt with Dλ (Amp R) This work pJET-Dλ pJET1.2/blunt with Dλ and 6xHis Tag (Amp R) This work pJET-Dλ-ZEDIII-L-6xHis pJET1.2/blunt with Dλ-ZEDIII-L fusion and 6xHis Tag This work pKQV4 Ptac promoter (Amp R) Strauch et al. 1989 pDλ-ZEDIII-6xHis pKQV4 with Dλ-ZEDIII fusion and 6xHis Tag (Amp R) This work pDλ-ZEDIII-L-6xHis pDλ-ZEDIII-6xHis with long linker (Amp R) This work pDλ-ZEDIII pKQV4 with Dλ-ZEDIII (Amp R) This work pDλ-TD-6xHis pKQV4 with Dλ fused to 3 different epitopes from EZIKV, separated by short linkers and 6xHis Tag (AmpR) This work pDλ-GFP pKQV4 with Dλ-GFP (Amp R) This work pDλ-GFP-L pDλ-GFP with long linker (Amp R) This work pDλ pKQV4 with Dλ (Amp R) This work pDλ-6xHis pKQV4 with Dλ and 6xHis Tag (Amp R) This work pKD46 pBAD promoter exo beta gam genes (CmR) Datsenko and Wanner 2000 pE-FLP pBAD promoter flp gene (CmR) Datsenko and Wanner 2000 pRSET-ZIKV/ES pRSET-A with EZIKV coding sequence corresponding to residues 1 to 409 (AmpR) This work † The SoluBL21 strain contains uncharacterized mutations which were obtained through special selection criteria. ..

    Sequencing:

    Article Title: A Lambda-evo (λ evo ) phage platform for Zika virus E DIII protein display
    Article Snippet: .. Escherichia coli K-12 derivative strains Genotype Reference DH5α supE44 D(lacIZYA-argF)U169 deoR (Φ80lacZDM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 Hanahan 1983 DH5α(λ) λ prophage This work DH5α(λΔD::KanR) λΔD::KanR prophage This work DH5α(λΔD) λΔD prophage This work W3110 F- λ - rph- Bachmann 1972 JW2787 F- Δ(araD-araB)567 ΔlacZ4787(::rrnB- 3) λ ΔrecD744::kan rph-1 Δ(rhaD-rhaB)568 hsdR514 Baba et al. 2006 SoluBL21† F- ompT hsdSB (rB- mB-) gal dcm (DE3) Deatherage et al. 2012 Bacteriophages/viral strains Genotype Reference λwt Lambda wild type CSH Collection λcI857 Lambda tscI857 CSH Collection λΔD::KanR λcI857 with a Kan R cassette inserted in D site This work λΔD λcI857 ΔD This work λevo λΔD with a 10,793 bp deletion in b region This work ZIKV Zika virus MEX_ENCB165P4 This work Plasmid Relevant markers Reference pJET1.2/blunt Eco471R (AmpR) Thermo Fisher Scientific 2024 pJET-ZEDIII-6xHis pJET1.2/blunt with ZEDIII coding sequence and 6xHis Tag (AmpR) This work pJET-Dλ-6xHis pJET1.2/blunt with Dλ (Amp R) This work pJET-Dλ pJET1.2/blunt with Dλ and 6xHis Tag (Amp R) This work pJET-Dλ-ZEDIII-L-6xHis pJET1.2/blunt with Dλ-ZEDIII-L fusion and 6xHis Tag This work pKQV4 Ptac promoter (Amp R) Strauch et al. 1989 pDλ-ZEDIII-6xHis pKQV4 with Dλ-ZEDIII fusion and 6xHis Tag (Amp R) This work pDλ-ZEDIII-L-6xHis pDλ-ZEDIII-6xHis with long linker (Amp R) This work pDλ-ZEDIII pKQV4 with Dλ-ZEDIII (Amp R) This work pDλ-TD-6xHis pKQV4 with Dλ fused to 3 different epitopes from EZIKV, separated by short linkers and 6xHis Tag (AmpR) This work pDλ-GFP pKQV4 with Dλ-GFP (Amp R) This work pDλ-GFP-L pDλ-GFP with long linker (Amp R) This work pDλ pKQV4 with Dλ (Amp R) This work pDλ-6xHis pKQV4 with Dλ and 6xHis Tag (Amp R) This work pKD46 pBAD promoter exo beta gam genes (CmR) Datsenko and Wanner 2000 pE-FLP pBAD promoter flp gene (CmR) Datsenko and Wanner 2000 pRSET-ZIKV/ES pRSET-A with EZIKV coding sequence corresponding to residues 1 to 409 (AmpR) This work † The SoluBL21 strain contains uncharacterized mutations which were obtained through special selection criteria. ..

    Article Title: A MultiSite Gateway Toolkit for Rapid Cloning of Vertebrate Expression Constructs with Diverse Research Applications
    Article Snippet: .. Unless otherwise stated, all 3’ entry vectors were generated by PCR amplification of the desired 3’ element using attB2R/B3-flanked oligonucleotide primers, followed by a BP reaction with pDONR P2R-P3 (Invitrogen). p3E-GFP-HA, p3E-YFP-HA (from pEYFP-C1, Clontech), p3E-CFP-HA (from pECFP-C1, Clontech), p3E-mCherry-HA (from p3E-mCherrypA [ ]), p3E-mKate2-HA and p3E-mKate2-myc no-polyadenylation signal (pA) were made by amplification of the coding sequences without a stop codon, but with the 3’ primer additionally containing an HA or c-myc epitope sequence followed by a stop codon. p3E-HA-Neuroligin1 was generated by amplification of HA-Neuroligin1 from a previously described vector [ ]. p3E-Dam-myc no-pA is from pNDam-Myc [ ]. p3E-SGTAP no-pA originated from pCeMM-CTAP(SG)-Gw [ ]. p3E-FRT-Kan R -FRT pA was cloned by amplifying the Kan R cassette from pCRII (ThermoFisher) with FRT sites in the 5’ and 3’ primers and inserting into the BamHI site of p3E-polyA [ ]. .. FRB variants and FKBP cassettes were amplified from Gerald Crabtree lab plasmids [ – ] to generate p3E-FRB-HA, p3E-FRB(PLF)-HA, p3E-FRB(KTF)-HA, p3E-FKBP-HA and p3E-mCherryFRB-HA no-pA. p3E-HA was made by cloning annealed oligos (5’-GATCCACTCGAGTATCCGTACGACGTACCAGACTACGCAGCATAGGGTACCAGTACTAAC-3’ and 5’-AATTGTTAGTACTGGTACCCTATGCTGCGTAGTCTGGTACGTCGTACGGATACTCGAGTG-3’) into BamHI/MfeI-digested p3E-polyA.

    Selection:

    Article Title: A Lambda-evo (λ evo ) phage platform for Zika virus E DIII protein display
    Article Snippet: .. Escherichia coli K-12 derivative strains Genotype Reference DH5α supE44 D(lacIZYA-argF)U169 deoR (Φ80lacZDM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 Hanahan 1983 DH5α(λ) λ prophage This work DH5α(λΔD::KanR) λΔD::KanR prophage This work DH5α(λΔD) λΔD prophage This work W3110 F- λ - rph- Bachmann 1972 JW2787 F- Δ(araD-araB)567 ΔlacZ4787(::rrnB- 3) λ ΔrecD744::kan rph-1 Δ(rhaD-rhaB)568 hsdR514 Baba et al. 2006 SoluBL21† F- ompT hsdSB (rB- mB-) gal dcm (DE3) Deatherage et al. 2012 Bacteriophages/viral strains Genotype Reference λwt Lambda wild type CSH Collection λcI857 Lambda tscI857 CSH Collection λΔD::KanR λcI857 with a Kan R cassette inserted in D site This work λΔD λcI857 ΔD This work λevo λΔD with a 10,793 bp deletion in b region This work ZIKV Zika virus MEX_ENCB165P4 This work Plasmid Relevant markers Reference pJET1.2/blunt Eco471R (AmpR) Thermo Fisher Scientific 2024 pJET-ZEDIII-6xHis pJET1.2/blunt with ZEDIII coding sequence and 6xHis Tag (AmpR) This work pJET-Dλ-6xHis pJET1.2/blunt with Dλ (Amp R) This work pJET-Dλ pJET1.2/blunt with Dλ and 6xHis Tag (Amp R) This work pJET-Dλ-ZEDIII-L-6xHis pJET1.2/blunt with Dλ-ZEDIII-L fusion and 6xHis Tag This work pKQV4 Ptac promoter (Amp R) Strauch et al. 1989 pDλ-ZEDIII-6xHis pKQV4 with Dλ-ZEDIII fusion and 6xHis Tag (Amp R) This work pDλ-ZEDIII-L-6xHis pDλ-ZEDIII-6xHis with long linker (Amp R) This work pDλ-ZEDIII pKQV4 with Dλ-ZEDIII (Amp R) This work pDλ-TD-6xHis pKQV4 with Dλ fused to 3 different epitopes from EZIKV, separated by short linkers and 6xHis Tag (AmpR) This work pDλ-GFP pKQV4 with Dλ-GFP (Amp R) This work pDλ-GFP-L pDλ-GFP with long linker (Amp R) This work pDλ pKQV4 with Dλ (Amp R) This work pDλ-6xHis pKQV4 with Dλ and 6xHis Tag (Amp R) This work pKD46 pBAD promoter exo beta gam genes (CmR) Datsenko and Wanner 2000 pE-FLP pBAD promoter flp gene (CmR) Datsenko and Wanner 2000 pRSET-ZIKV/ES pRSET-A with EZIKV coding sequence corresponding to residues 1 to 409 (AmpR) This work † The SoluBL21 strain contains uncharacterized mutations which were obtained through special selection criteria. ..

    Generated:

    Article Title: A MultiSite Gateway Toolkit for Rapid Cloning of Vertebrate Expression Constructs with Diverse Research Applications
    Article Snippet: .. Unless otherwise stated, all 3’ entry vectors were generated by PCR amplification of the desired 3’ element using attB2R/B3-flanked oligonucleotide primers, followed by a BP reaction with pDONR P2R-P3 (Invitrogen). p3E-GFP-HA, p3E-YFP-HA (from pEYFP-C1, Clontech), p3E-CFP-HA (from pECFP-C1, Clontech), p3E-mCherry-HA (from p3E-mCherrypA [ ]), p3E-mKate2-HA and p3E-mKate2-myc no-polyadenylation signal (pA) were made by amplification of the coding sequences without a stop codon, but with the 3’ primer additionally containing an HA or c-myc epitope sequence followed by a stop codon. p3E-HA-Neuroligin1 was generated by amplification of HA-Neuroligin1 from a previously described vector [ ]. p3E-Dam-myc no-pA is from pNDam-Myc [ ]. p3E-SGTAP no-pA originated from pCeMM-CTAP(SG)-Gw [ ]. p3E-FRT-Kan R -FRT pA was cloned by amplifying the Kan R cassette from pCRII (ThermoFisher) with FRT sites in the 5’ and 3’ primers and inserting into the BamHI site of p3E-polyA [ ]. .. FRB variants and FKBP cassettes were amplified from Gerald Crabtree lab plasmids [ – ] to generate p3E-FRB-HA, p3E-FRB(PLF)-HA, p3E-FRB(KTF)-HA, p3E-FKBP-HA and p3E-mCherryFRB-HA no-pA. p3E-HA was made by cloning annealed oligos (5’-GATCCACTCGAGTATCCGTACGACGTACCAGACTACGCAGCATAGGGTACCAGTACTAAC-3’ and 5’-AATTGTTAGTACTGGTACCCTATGCTGCGTAGTCTGGTACGTCGTACGGATACTCGAGTG-3’) into BamHI/MfeI-digested p3E-polyA.

    Amplification:

    Article Title: A MultiSite Gateway Toolkit for Rapid Cloning of Vertebrate Expression Constructs with Diverse Research Applications
    Article Snippet: .. Unless otherwise stated, all 3’ entry vectors were generated by PCR amplification of the desired 3’ element using attB2R/B3-flanked oligonucleotide primers, followed by a BP reaction with pDONR P2R-P3 (Invitrogen). p3E-GFP-HA, p3E-YFP-HA (from pEYFP-C1, Clontech), p3E-CFP-HA (from pECFP-C1, Clontech), p3E-mCherry-HA (from p3E-mCherrypA [ ]), p3E-mKate2-HA and p3E-mKate2-myc no-polyadenylation signal (pA) were made by amplification of the coding sequences without a stop codon, but with the 3’ primer additionally containing an HA or c-myc epitope sequence followed by a stop codon. p3E-HA-Neuroligin1 was generated by amplification of HA-Neuroligin1 from a previously described vector [ ]. p3E-Dam-myc no-pA is from pNDam-Myc [ ]. p3E-SGTAP no-pA originated from pCeMM-CTAP(SG)-Gw [ ]. p3E-FRT-Kan R -FRT pA was cloned by amplifying the Kan R cassette from pCRII (ThermoFisher) with FRT sites in the 5’ and 3’ primers and inserting into the BamHI site of p3E-polyA [ ]. .. FRB variants and FKBP cassettes were amplified from Gerald Crabtree lab plasmids [ – ] to generate p3E-FRB-HA, p3E-FRB(PLF)-HA, p3E-FRB(KTF)-HA, p3E-FKBP-HA and p3E-mCherryFRB-HA no-pA. p3E-HA was made by cloning annealed oligos (5’-GATCCACTCGAGTATCCGTACGACGTACCAGACTACGCAGCATAGGGTACCAGTACTAAC-3’ and 5’-AATTGTTAGTACTGGTACCCTATGCTGCGTAGTCTGGTACGTCGTACGGATACTCGAGTG-3’) into BamHI/MfeI-digested p3E-polyA.

    Clone Assay:

    Article Title: A MultiSite Gateway Toolkit for Rapid Cloning of Vertebrate Expression Constructs with Diverse Research Applications
    Article Snippet: .. Unless otherwise stated, all 3’ entry vectors were generated by PCR amplification of the desired 3’ element using attB2R/B3-flanked oligonucleotide primers, followed by a BP reaction with pDONR P2R-P3 (Invitrogen). p3E-GFP-HA, p3E-YFP-HA (from pEYFP-C1, Clontech), p3E-CFP-HA (from pECFP-C1, Clontech), p3E-mCherry-HA (from p3E-mCherrypA [ ]), p3E-mKate2-HA and p3E-mKate2-myc no-polyadenylation signal (pA) were made by amplification of the coding sequences without a stop codon, but with the 3’ primer additionally containing an HA or c-myc epitope sequence followed by a stop codon. p3E-HA-Neuroligin1 was generated by amplification of HA-Neuroligin1 from a previously described vector [ ]. p3E-Dam-myc no-pA is from pNDam-Myc [ ]. p3E-SGTAP no-pA originated from pCeMM-CTAP(SG)-Gw [ ]. p3E-FRT-Kan R -FRT pA was cloned by amplifying the Kan R cassette from pCRII (ThermoFisher) with FRT sites in the 5’ and 3’ primers and inserting into the BamHI site of p3E-polyA [ ]. .. FRB variants and FKBP cassettes were amplified from Gerald Crabtree lab plasmids [ – ] to generate p3E-FRB-HA, p3E-FRB(PLF)-HA, p3E-FRB(KTF)-HA, p3E-FKBP-HA and p3E-mCherryFRB-HA no-pA. p3E-HA was made by cloning annealed oligos (5’-GATCCACTCGAGTATCCGTACGACGTACCAGACTACGCAGCATAGGGTACCAGTACTAAC-3’ and 5’-AATTGTTAGTACTGGTACCCTATGCTGCGTAGTCTGGTACGTCGTACGGATACTCGAGTG-3’) into BamHI/MfeI-digested p3E-polyA.



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    New England Biolabs kan r cassette
    Kan R Cassette, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/kan+r+cassette/pmc06163455-118-30-40
    Average 86 stars, based on 1 article reviews
    kan r cassette - by Bioz Stars, 2026-10
    86/100 stars
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    95
    Thermo Fisher kan r cassette
    Kan R Cassette, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/kan+r+cassette/Kanamycin+monosulfate/pmc12356441-33-27-50
    Average 95 stars, based on 1 article reviews
    kan r cassette - by Bioz Stars, 2026-10
    95/100 stars
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    90
    Genoscope tdk/kan r cassette
    Tdk/Kan R Cassette, supplied by Genoscope, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/kan+r+cassette/tdk+kan+r+cassette/pmc11075230-107-1-25
    Average 90 stars, based on 1 article reviews
    tdk/kan r cassette - by Bioz Stars, 2026-10
    90/100 stars
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    90
    Gene Bridges Inc galff‐polya‐kan r cassette
    Galff‐Polya‐Kan R Cassette, supplied by Gene Bridges Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/kan+r+cassette/galff+polya+kan+r+cassette/pmc07754311-127-15-22
    Average 90 stars, based on 1 article reviews
    galff‐polya‐kan r cassette - by Bioz Stars, 2026-10
    90/100 stars
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    95
    Thermo Fisher kan r cassettes
    Kan R Cassettes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/kan+r+cassette/Kanamycin+monosulfate/pmc03605328-93-8-13
    Average 95 stars, based on 1 article reviews
    kan r cassettes - by Bioz Stars, 2026-10
    95/100 stars
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    99
    ATCC tet r resistance cassette 30 prn5101 δ bcla atcc 14579 kan r kan r
    Tet R Resistance Cassette 30 Prn5101 δ Bcla Atcc 14579 Kan R Kan R, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/kan+r+cassette/Bacillus+cereus+Frankland+and+Frankland/pmc03147369-147-175-183
    Average 99 stars, based on 1 article reviews
    tet r resistance cassette 30 prn5101 δ bcla atcc 14579 kan r kan r - by Bioz Stars, 2026-10
    99/100 stars
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